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"I am involved in resequencing projects for disease association studies. Sequencher was of tremendous help to me in these projects for- discovery of SNPs, detection of known SNPs, intronic/exonic SNPs & amino acid changing SNPs. I have been using it for sequence analysis of ABI data files since 2000. It is easy to operate and user friendly. It was a pleasure to be involved in the beta test program for version 4.6. What I liked in this new version is assembly function & variance table. Assemble to reference by defining assembly parameters using advance expression feature is very useful, it saves a lot of time and sequences can be assembled by defining feature of your choice e.g. sample ID, primer ID, or well position etc. Technical Support group at the Gene Codes Corporation has been very receptive to the idea of introducing variance table. With this feature it is now possible to export genotypes. Using this feature I am not only able to export whole table but also selected few columns using “export selected” option button. "